Hi All,
I am new to DESeq2 analysis and I follow the trinity pipeline for DESeq2 analysis. In that pipeline, RSEM is used to quantify the transcript abundance which generates the expected counts. These expected counts will be rounded off and later fed into DESeq2 pipeline for further analysis.
I would like to know whether these expected counts generated from RSEM can be fed into DESeq2 instead of raw counts for computing differential expressed genes?
Regards
Chudar

