Transform data from MoFlo Astrios
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cr • 0
@cr-12159
Last seen 2.7 years ago

Hello,

I have different fcs datafile from MoFlo Astrios, and I want to transform it into log.

I read the data with "read.FCS" from "flowCore" package but I haven't yet find a way to transform my data.

How can I do it ? Thank you

Céline

transform flow cytometry data • 664 views
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SamGG ▴ 270
@samgg-6428
Last seen 9 weeks ago
France/Marseille/Inserm

Hi,

Look at the function logTransform function and its example.

?logTransform

 

samp <- read.FCS(system.file("extdata",
   "0877408774.B08", package="flowCore"))
logTrans <- logTransform(transformationId="log10-transformation", logbase=10, r=1, d=1)
trans <- transformList('FSC-H', logTrans)
dataTransform <- transform(samp, trans)

 

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