limma example Weaver Data
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Wenbin Liu ▴ 100
@wenbin-liu-943
Last seen 6.8 years ago
Dear Gordon and limma users, I'm struggling to understand the design matrices in the Weaver Data in the Case Studies section of the limma User's Guide. In the single loop design, there are 4 arrays and 4 conditions (from two time points and two genotypes). I tried using modelMatrix() with the target from the example and assigning ref='P11WT'. I would like to check the effect 'TimeWt' which is the late vs. early for WT. Unfortunately, I have been unable to get Gordon's result (topTable result). Can someone show me how to get Gordon's design matrices (two of them in that example) by using the function modelMatrix() and probably plus makeContrasts()? Thanks in advance! Sincerely, Wenbin
limma weaver limma weaver • 797 views
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@gordon-smyth
Last seen 14 minutes ago
WEHI, Melbourne, Australia
I guess that you are using an older version of the software. The Weaver example was expanded and simplified last October, and full commands are now given to compute the design matrix, including use of modelMatrix() and makeContrasts(). Gordon ------ original message -------- [BioC] limma example Weaver Data Wenbin Liu wnbnl at yahoo.com Thu May 26 23:25:09 CEST 2005 Dear Gordon and limma users, I'm struggling to understand the design matrices in the Weaver Data in the Case Studies section of the limma User's Guide. In the single loop design, there are 4 arrays and 4 conditions (from two time points and two genotypes). I tried using modelMatrix() with the target from the example and assigning ref='P11WT'. I would like to check the effect 'TimeWt' which is the late vs. early for WT. Unfortunately, I have been unable to get Gordon's result (topTable result). Can someone show me how to get Gordon's design matrices (two of them in that example) by using the function modelMatrix() and probably plus makeContrasts()? Thanks in advance! Sincerely, Wenbin
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Thanks, Gordon! In the expanded example, you have a common RNA source, the Pool. What if one doesn't have a common RNA source, such as when it is a loop design with more than three arrays like the unexpanded Weaver data? Can you please show me how to generate the matrices? Thanks! Wenbin --- Gordon K Smyth <smyth@wehi.edu.au> wrote: > I guess that you are using an older version of the > software. The Weaver example was expanded and > simplified last October, and full commands are now > given to compute the design matrix, including > use of modelMatrix() and makeContrasts(). > > Gordon > > ------ original message -------- > [BioC] limma example Weaver Data > Wenbin Liu wnbnl at yahoo.com > Thu May 26 23:25:09 CEST 2005 > > Dear Gordon and limma users, > > I'm struggling to understand the design matrices in > the Weaver Data in the Case Studies section of the > limma User's Guide. > > In the single loop design, there are 4 arrays and 4 > conditions (from two time points and two genotypes). > I tried using modelMatrix() with the target from the > example and assigning ref='P11WT'. I would like to > check the effect 'TimeWt' which is the late vs. > early > for WT. Unfortunately, I have been unable to get > Gordon's result (topTable result). > > Can someone show me how to get Gordon's design > matrices (two of them in that example) by using the > function modelMatrix() and probably plus > makeContrasts()? > > Thanks in advance! > > Sincerely, > Wenbin > > > >
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Wenbin, I have found this link quite useful for figuring out design matrix for direct designs: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi? cmd=Retrieve&db=PubMed&list_uids=14744830&dopt=Abstract Sean On May 31, 2005, at 11:32 AM, Wenbin Liu wrote: > Thanks, Gordon! In the expanded example, you have a > common RNA source, the Pool. What if one doesn't have > a common RNA source, such as when it is a loop design > with more than three arrays like the unexpanded Weaver > data? Can you please show me how to generate the > matrices? Thanks! > > > Wenbin > > --- Gordon K Smyth <smyth@wehi.edu.au> wrote: > >> I guess that you are using an older version of the >> software. The Weaver example was expanded and >> simplified last October, and full commands are now >> given to compute the design matrix, including >> use of modelMatrix() and makeContrasts(). >> >> Gordon >> >> ------ original message -------- >> [BioC] limma example Weaver Data >> Wenbin Liu wnbnl at yahoo.com >> Thu May 26 23:25:09 CEST 2005 >> >> Dear Gordon and limma users, >> >> I'm struggling to understand the design matrices in >> the Weaver Data in the Case Studies section of the >> limma User's Guide. >> >> In the single loop design, there are 4 arrays and 4 >> conditions (from two time points and two genotypes). >> I tried using modelMatrix() with the target from the >> example and assigning ref='P11WT'. I would like to >> check the effect 'TimeWt' which is the late vs. >> early >> for WT. Unfortunately, I have been unable to get >> Gordon's result (topTable result). >> >> Can someone show me how to get Gordon's design >> matrices (two of them in that example) by using the >> function modelMatrix() and probably plus >> makeContrasts()? >> >> Thanks in advance! >> >> Sincerely, >> Wenbin >> >> >> >> > > _______________________________________________ > Bioconductor mailing list > Bioconductor@stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor
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