I am working with data obtained from GEO (NCBI). This data is gene expression found with the Affymetrix Human Genome U133A Array and is analysed to get a log2 from the raw data (fastlo normalized, rma summarized). Could you help me understand what this data is and how I should plot it? I thought that I should just plot it normally, but my PI tells me it is possible that I should put it in a logarithmic scale.
I am trying to look at different genes (7) to find if they are up-regulated or down-regulated in a disease and using actin and TLR4 as max and min expression. Which graph would be best for this and how should I go about this?
Thanks in advance, Lara.