Hi,

I have been trying to import a bigwig file into RStudio. It worked for me the first two times I tried importing the files, however, after that, I was met with an error message provided below. After reading about possible solutions, I also used the terminal. However, I receive the same error message. Please let me know how can I address this problem.

Importing bigwig files

Sample1 <- rtracklayer::import.bw("/Users/sukritmahajan/Desktop/All_Tracks/01_AC1_inp_S1_mapq3.bw")

Error message received

Error in seqinfo(ranges) : UCSC library operation failed In addition: Warning message: In seqinfo(ranges) : No such file or directory mustOpenFd: Can't open /Users/sukritmahajan/Desktop/All_Tracks/01_AC1_inp_S1_mapq3.bw to read```