Hello

I don't know what is happening but I am getting a permanent error in DESeq2

> dds <- DESeqDataSetFromMatrix(countData=d, 
+                                colData=metaData, 
+                                design=~condition)
Error in h(simpleError(msg, call)) : 
  error in evaluating the argument 'x' in selecting a method for function 'nrow': object 'metaData' not found
> 

I can not find the solution

Help me please

I tried as.data.frame(d)

and as.matrix(d) but does not help

I am sure row names of metadata and colnames of d is the same

Hey Fereshteh,

It seems that you have not yet initialised the metaData object. Can you show your commands prior to running DESeqDataSetFromMatrix()?

Note the difference between 'metaData' and 'metadata' ('D' versus' 'd')

Kind regards,

Kevin