I would like to perform a pair analysis in
Deseq2 and I read the vignette and previous questions here but still I'm not sure if my matrix is correct.
SampleID Factor Condition Replicate sample1 eprint siNeg 1 sample2 eprint siNeg 2 sample3 eprint siFUS 1 sample4 eprint siFUS 2 sample5 input siNeg 1 sample6 input siNeg 2 sample7 input siFUS 1 sample8 input siFUS 2
Consider the following table, I would like to have the following pairs :
I'm not sure if my
Replicate column is correctly accounting for the paired samples if coded this way. Ultimately my final model should be the following: