Hello, Is it correct to use the variance stabilizing transformation from DESeq2 to normalize bulk UMI counts? I have a matrix samplesXgenes with UMI counts and i was wondering if DESeq2 can handle UMI counts too or only read counts.
This is what i'm simply doing:
dds <- DESeqDataSetFromMatrix(countData=as.matrix(umi_matrix), colData=targets, design=~subtype) dds <- dds[rowSums(counts(dds))>=10,] vsd <- varianceStabilizingTransformation(dds, blind=F)
Thanks in advance for any help!