I would like to know if you could help me about someting in DESeq2.
In the documentation, they talk about technical replicates ad biological replicates. From what I understand:
- biological replicates are for example samples that do not come from the same people/animal/cellular culture.
- technical replicates are for example samples that were sequenced twice on the same or on different machine.
After discussion with other bioinformatician and biologist, it appears to me that sometime we do not have the same consideration of the term "biological replicate". Indeed, If I make some swab from the same animal, does it results in technical replicate (because they come from the same individual) or biological replicates ? (because it's not exactly the same sample that is sequenced twice).
I hope my question is enough clear.