Question about my design
1
0
Entering edit mode
leo.dagata • 0
@bcdc7141
Last seen 5 days ago
France

Hello everyone,

I would like to know if you could help me about knowing if I can use DESeq2 for the following design:

I have only 1 animal that has 12 timepoints. The 6 first timepoints are "before infection". The 6 last timepoints are "after infection".

Is it possible and relevant for you if I use DESeq2 to compare the 6 samples before infection (group A) versus the 6 samples after infection (group B) even if I have only one animal ? If yes, do you recommend to had also the "timepoints" variable in the DESeq2 model ?

I hope my question is enough clear.

Thanks

Léo

DESeq2 • 79 views
ADD COMMENT
0
Entering edit mode
@mikelove
Last seen 1 day ago
United States

You can just use a two group comparison. Your inference will not generalize to other animals obviously, because you are only comparing within one animal.

ADD COMMENT

Login before adding your answer.

Traffic: 161 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6