ATACseqQC function fragSizeDist returns error
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Entering edit mode
@xuyao15927402897-20826
Last seen 2.6 years ago
Germany

Species: wheat

There is no error message when using bam files of other species as input, The following is the error message.

Error in value[[3L]](cond) : 'end' must be <= 536870912
  file: ../mapping/CT_2_1.uni.dedup.bam
  index: ../mapping/CT_2_1.uni.dedup.bam
Calls: fragSizeDist ... tryCatch -> tryCatchList -> tryCatchOne -> <Anonymous>


# include your problematic code here with any corresponding output 
# please also include the results of running the following in an R session 

sessionInfo( )
R version 3.5.1 (2018-07-02)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)

Matrix products: default
BLAS: /PUBLIC/software/RNA/R/R-3.5.1/lib64/R/lib/libRblas.so
LAPACK: /PUBLIC/software/RNA/R/R-3.5.1/lib64/R/lib/libRlapack.so

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
[1] ATACseqQC_1.6.4     S4Vectors_0.20.1    BiocGenerics_0.28.0

loaded via a namespace (and not attached):
 [1] ProtGenerics_1.14.0           bitops_1.0-6                 
 [3] matrixStats_0.55.0            bit64_0.9-7                  
 [5] progress_1.2.2                httr_1.4.1                   
 [7] GenomeInfoDb_1.18.2           tools_3.5.1                  
 [9] R6_2.4.1                      rGADEM_2.30.0                
[11] KernSmooth_2.23-16            seqLogo_1.48.0               
[13] DBI_1.1.0                     lazyeval_0.2.2               
[15] colorspace_1.4-0              ade4_1.7-15                  
[17] motifStack_1.26.0             prettyunits_1.1.1            
[19] bit_1.1-15.2                  curl_4.3                     
[21] compiler_3.5.1                VennDiagram_1.6.20           
[23] graph_1.60.0                  Biobase_2.42.0               
[25] formatR_1.7                   DelayedArray_0.8.0           
[27] grImport_0.9-3                rtracklayer_1.42.2           
[29] scales_1.0.0                  randomForest_4.6-14          
[31] RBGL_1.58.2                   stringr_1.4.0                
[33] digest_0.6.18                 Rsamtools_1.34.1             
[35] XVector_0.22.0                pkgconfig_2.0.3              
[37] htmltools_0.4.0               fastmap_1.0.1                
[39] ensembldb_2.6.8               limma_3.38.3                 
[41] BSgenome_1.50.0               regioneR_1.14.0              
[43] htmlwidgets_1.5.1             rlang_0.4.8                  
[45] RSQLite_2.2.0                 shiny_1.4.0                  
[47] BiocParallel_1.16.6           RCurl_1.98-1.1               
[49] magrittr_1.5                  polynom_1.4-0                
[51] GO.db_3.7.0                   GenomeInfoDbData_1.2.0       
[53] futile.logger_1.4.3           Matrix_1.2-15                
[55] Rcpp_1.0.3                    munsell_0.5.0                
[57] stringi_1.4.6                 yaml_2.2.1                   
[59] MASS_7.3-51.1                 SummarizedExperiment_1.12.0  
[61] zlibbioc_1.28.0               AnnotationHub_2.14.5         
[63] grid_3.5.1                    blob_1.2.1                   
[65] promises_1.1.0                crayon_1.3.4                 
[67] lattice_0.20-38               Biostrings_2.50.2            
[69] splines_3.5.1                 multtest_2.38.0              
[71] GenomicFeatures_1.34.8        hms_0.5.3                    
[73] GenomicScores_1.6.0           MotIV_1.38.0                 
[75] GenomicRanges_1.34.0          seqinr_3.6-1                 
[77] biomaRt_2.38.0                futile.options_1.0.1         
[79] XML_3.98-1.16                 lambda.r_1.2.4               
[81] BiocManager_1.30.10           idr_1.2                      
[83] vctrs_0.3.4                   httpuv_1.5.2                 
[85] mime_0.9                      preseqR_4.0.0                
[87] xtable_1.8-4                  AnnotationFilter_1.6.0       
[89] later_1.0.0                   survival_3.1-8               
[91] ChIPpeakAnno_3.16.1           GenomicAlignments_1.18.1     
[93] AnnotationDbi_1.44.0          memoise_1.1.0                
[95] IRanges_2.16.0                interactiveDisplayBase_1.20.0

Thanks,

Yao

2021-8-28

fragSizeDist ATACseqQC • 441 views
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