Hi all, I am a wet lab scientist trying to understand how a DEGUST works... I read a few things about normalization but I still don't grasp 100% of the methods that are out there.
I am using DEGUST to look at some data and I am using the Voom/Limma method. DEGUST gives me normalised counts in CPM. But what is the actual normalization method used? I am going along using this paper... so is it normalisation by distribution it uses or by library size?
Any help to understand this is highly appreciated! Thank you!