Degust: what normalization method does it use
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Caroline • 0
@478c0714
Last seen 29 days ago
United Kingdom

Hi all, I am a wet lab scientist trying to understand how a DEGUST works... I read a few things about normalization but I still don't grasp 100% of the methods that are out there.

I am using DEGUST to look at some data and I am using the Voom/Limma method. DEGUST gives me normalised counts in CPM. But what is the actual normalization method used? I am going along using this paper... so is it normalisation by distribution it uses or by library size?

Any help to understand this is highly appreciated! Thank you!

DEGUST Normalization CPM • 118 views
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@james-w-macdonald-5106
Last seen 3 hours ago
United States

The 'stock' method of normalization for limma-voom is to compute logCPM using library sizes that are adjusted using a TMM normalization. The paper describing TMM can be found here.

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