is it possible to use TPM in DESeq2 ?
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Hicham • 0
@2952f756
Last seen 10 days ago
Morocco

My question is quite simple, I want to know is it possible to use TPM data to normalize them with DESeq2? and why?

counts Normalization DESeq2 • 197 views
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@kevin
Last seen 1 day ago
V&A Waterfront, Cape Town, South Africa

No. DESeq2 requires raw count data as input.

If you would like to use TPM, then please take a look at these previous answers, which additionally link to other answers:

Kevin

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I found that data I downloaded is quantified using RSEM is it also not directly useful for DESeq2 ?

the description related to my data "tab-delimited data matrix TPM quantified by RSEM for all samples"

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Expected counts from RSEM is fine for DESeq. With tximport, you can put the RSEM input into DESeq, and it will even apply offsets for gene length.

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Ah ha, yes, coming via the tximport route and RSEM is okay.

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But it sounds like the OP only has the TPM pulled from RSEM, and not the whole output.

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