In my dataset, the number of samples from each individual are different, something like this:
I want to compare active and inactive status, but as you can see, patient 1,2,3 has 1 samples in each status, whereas patient 4,5,6,7,8 contribute multiple samples in each status.
In this scenario, can I design my Deseq model as:
~ status + Patient.ID
to eliminate the unequal variance issue?