Using featureCounts and downloading Rsubread
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Entering edit mode
margo • 0
@4769e097
Last seen 5 weeks ago
United Kingdom

I am trying to perform a count per gene analysis using featureCounts in R. I have downloaded the gtf file and edited it within R to only contain the gene ID, chr, start, end, and strand, within a data frame (df2). I am trying to run featureCounts on df2 with my 6 bam files, however, I am unable to download the package Rsubread.

library("Rsubread")
Error in library("Rsubread") : there is no package called ‘Rsubread’

The code I have within my R script so far is:

install.packages('BiocManager')
library(BiocManager)
install()
library('Rsubread')
featureCounts RNASeq Rsubread R • 122 views
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1
Entering edit mode
@james-w-macdonald-5106
Last seen 8 hours ago
United States

You need to tell BiocManager;;install that you want to install the Rsubread package.

BiocManager::install("Rsubread")
library(Rsubread)

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