I have run the ASICS package to analyse H-NMR spectra from urine samples. I have correctly obtained the relative concentration of multiple metabolites. However, I would like now to add the internal standard spectra in the pure spectra library in order to calculate the absolute concentration. I am using TMSP, which has a ppm = 0. If I am not wrong, the spectra analysis starts at 0.5 ppm, so I cannot obtain the data refering to TMSP peak. Is there any way to solve this issue?
Many thanks in advance!