**0**wrote:

Hi,

Normalized counts from the matrix table are transformed to log2 values by metagenomeseq, aren´t they? My question is about the zeros in the count matrix. How are they transformed to allow statistical analysis? (as log2 of zero gives an error). Thanks!

**280**• written 2.8 years ago by PJ •

**0**

In addition, how is it possible that features with negative log2 values in the transformed count matrix come up with positive average expression values in the statistical output? Thanks!

0