I want to add the annotation row labelling to a pheatmap.

It appears from the tutorial here: https://towardsdatascience.com/pheatmap-draws-pretty-heatmaps-483dab9a3cc

That I can call a data frame in order to do this.

Here is my data frame:

```
Sample Condition
1 Sample_Run1HR62_S1_Run1 groupa
2 Sample_Run2HR62_S1_Run2 groupa
3 Sample_Run1HR70_S2_Run1 groupa
4 Sample_Run2HR70_S2_Run2 groupa
5 Sample_Run1HR78_S3_Run1 groupa
6 Sample_Run2HR78_S3_Run2 groupa
7 Sample_Run1HR81_S4_Run1 groupa
8 Sample_Run2HR81_S4_Run2 groupa
9 Sample_Run1HR87_S5_Run1 groupa
10 Sample_Run2HR87_S5_Run2 groupa
11 Sample_Run1HR99_S6_Run1 groupa
12 Sample_Run2HR99_S6_Run2 groupa
13 Sample_Run1HR107_S7_Run1 groupa
14 Sample_Run2HR107_S7_Run2 groupa
15 Sample_Run1HR114_S8_Run1 groupa
16 Sample_Run2HR114_S8_Run2 groupa
17 Sample_Run1HR142_S17_Run1 groupa
18 Sample_Run2HR142_S17_Run2 groupa
19 Sample_Run1HR146_S18_Run1 groupa
20 Sample_Run2HR146_S18_Run2 groupa
21 Sample_Run1HR61_S9_Run1 groupb
22 Sample_Run2HR61_S9_Run2 groupb
23 Sample_Run1HR71_S11_Run1 groupb
24 Sample_Run2HR71_S11_Run2 groupb
25 Sample_Run1HR74_S41_Run1 groupb
26 Sample_Run2HR74_S41_Run2 groupb
27 Sample_Run1HR80_S12_Run1 groupb
28 Sample_Run2HR80_S12_Run2 groupb
29 Sample_Run1HR86_S13_Run1 groupb
30 Sample_Run2HR86_S13_Run2 groupb
31 Sample_Run1HR115_S14_Run1 groupb
32 Sample_Run2HR115_S14_Run2 groupb
33 Sample_Run1HR121_S15_Run1 groupb
34 Sample_Run2HR121_S15_Run2 groupb
35 Sample_Run1HR127_S16_Run1 groupb
36 Sample_Run2HR127_S16_Run2 groupb
37 Sample_Run2HR66_S10_Run2 groupb
38 Sample_Run1HR66_S10_Run1 groupb
```

Here is the r script I am using to generate the Pheatmap:

```
# Create sample-sample heatmap
sampleDists <- dist(t(assay(rld))) #calculates Euclidean distance. Rld to ensure we have a roughly equal contribution from all genes
sampleDistMatrix <- as.matrix( sampleDists )
rownames(sampleDistMatrix) <- paste( targets$Sample, sep = " - " )
colnames(sampleDistMatrix) <- NULL
colors <- colorRampPalette( rev(brewer.pal(9, "Blues")) )(255)
pheatmap(sampleDistMatrix, clustering_distance_rows = sampleDists, clustering_distance_cols = sampleDists,col = colors, main = "Heatmap of Sample to Sample Distances in Pig Samples" )
```

Here is the same code when I add the 'annotation_row' command:

```
# Create sample-sample heatmap
sampleDists <- dist(t(assay(rld))) #calculates Euclidean distance. Rld to ensure we have a roughly equal contribution from all genes
sampleDistMatrix <- as.matrix( sampleDists )
rownames(sampleDistMatrix) <- paste( targets$Sample, sep = " - " )
colnames(sampleDistMatrix) <- NULL
colors <- colorRampPalette( rev(brewer.pal(9, "Blues")) )(255)
pheatmap(sampleDistMatrix, clustering_distance_rows = sampleDists, clustering_distance_cols = sampleDists,col = colors,annotation_row = targets, main = "Heatmap of Sample to Sample Distances in Pig Samples" )
```

Here is the error generated from this:

```
Error in check.length("fill") :
'gpar' element 'fill' must not be length 0
```

Any help would be greatly appreciated