DESeq2 on normalized data
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lizaaa • 0
@8aed39be
Last seen 17 days ago
Russia

I've recently started using DESeq2. Unfortunately, I don't have raw counts data but have normalized counts. How can I analyse gene expression for already normalized counts using DESeq2?

DESeq2 • 123 views
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ATpoint ★ 1.6k
@atpoint-13662
Last seen 23 hours ago
Germany

It’s not supported. Use limma-trend with the log2-transformed normalized counts if that is all you have. See limma manual.

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