Interpret MetaVolcanoR plot
0
0
Entering edit mode
JAcky • 0
@d6b8183e
Last seen 18 months ago
Israel

I have multiple datasets that I applied limma's differential expression analysis. To present the genes, I found that the MetaVolcanoR package is suitable. But, I don't understand how can I tune the parameters of the plot. How do I tell it what Mean Fold change (the x-axis) is significant? like how do I determine the threshold?

And the same for the P value ( y-axis), I see there is a threshold around 0.5 in this plot, how do I know for sure what threshold it's using?

This is my code: (the deg data frames are the results, containing all the genes for each data with the p-value and log fold change):

totalDEG = list(table4 = deg4, table6 = deg6,
                table7 = deg7,table8 = deg8 ,
                table15 = deg15, table21 = deg21)
totalDEG = map(totalDEG, ~ .x %>% rownames_to_column("symbol") %>% `row.names<-`(.$symbol))

meta_degs_comb <- combining_mv(diffexp=totalDEG,
                               pcriteria='adj.P.Val', 
                               foldchangecol='LogFC',
                               genenamecol='symbol',
                               geneidcol=NULL,
                               metafc='Mean',
                               metathr=0.03, 
                               collaps = TRUE,
                               jobname="MetaVolcano",
                               outputfolder=".",
                               draw='HTML')


plot(meta_degs_comb@MetaVolcano )+ ylab('-log10.Pval') 

The plot:

enter image description here

MetaVolcanoR • 554 views
ADD COMMENT
0
Entering edit mode

Removed EnhancedVolcano tag.

ADD REPLY

Login before adding your answer.

Traffic: 750 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6