Hi, I am doing the RNA-Seq data analysis of a non-model organism these days, and a few questions occurred to me. The non-model organism doesn't have a orgDb, so I have to use the org.Hs.eg.db. When we do ORA for gene function enrichment, we need the 'Gene Ratio' and 'Background Gene Ratio' to confirm the significance of a specific GO term or KEGG pathway. Since different species have different gene numbers, is the significance of a GO term based on the human 'Background Gene Ratio' still meaningful for the non-model organism?
Looking forward to your help!