I am working with a non model organism called Tenualosa ilisa. I am working with RNA-seq data. De novo assembly was performed using trinity. Foe kegg enrichment analysis, at first blastx was performed against kegg database. From blast result I found a output file where a column contain KO id. I also perform Differential expression of the transcripts. so I have two files one containing transcript id and KO id, and other is differentially expressed transcript result file. with these how can i go forward for kegg enrichment analysis? Can anyone please provide me the complete pipeline with coding and step by step process to do this?
Any advised would be much appreciated! Thanks in advance!