Hello,

I am a new user of the package NanoStringDiff. I am working on an experiment with 4 groups and I am trying to figure out how to design the contrast vector, **specifically which numbers to use for each group**. To the best of my understanding, "0" omits the group, and the numbers, "1,-1" are used in the examples of the package. Should I use "-1/2, 1/2" for the other 2 groups? Is there any chance that 1/2 takes the average of the corresponding group? In other words, how to decide which numbers to use for the contrast vector if we have more than 2 groups?

Thank you everyone

I hit Enter too soon on my post, so you will need to come to the support site to see the whole thing.