Dear colleagues, I need help.
I have no experience with microarray files deposited in GEO and I have some doubts.
I don't want to do a differential expression analysis, I want to name the genes and come up with an expression value for each one, in each sample. I intend to plot a heatmap graph of all samples for some genes. For the graphics I already have a script.
I made a "manual annotation", using the procv function of the spreadsheet and observed that some genes are represented by more than one probe, with different expression values. How do I analyze this type of data?
Another question is how do I annotate banks like GSE77930 in which the IDs of the probes in the file with expression values are different from the IDs in the identification file of the GPL21289 genes?
Thanks in advance to anyone willing to help me. Best regards,
Michele Breton ```