Error when running GDC_prepare
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@4bb5d1af
Last seen 6 months ago
Sweden

I'm trying to retrieve and prepare some data from GDC and I'm running the following code which I copy-pasted from this YouTube video: https://www.youtube.com/watch?v=UWXv9dUpxNE


library(TCGAbiolinks)
library(tidyverse)
library(maftools)
library(pheatmap)
library(SummarizedExperiment)


# get a list of projects
gdcprojects <- getGDCprojects()


# building a query
query_TCGA <- GDCquery(project = 'TCGA-BRCA',
                       data.category = 'Transcriptome Profiling')
output_query_TCGA <- getResults(query_TCGA)


# build a query to retrieve gene expression data ------------
query_TCGA <- GDCquery(project = 'TCGA-BRCA',
                       data.category = 'Transcriptome Profiling',
                       experimental.strategy = 'RNA-Seq',
                       workflow.type = 'STAR - Counts',
                       access = 'open',
                       barcode = c('TCGA-LL-A73Y-01A-11R-A33J-07', 'TCGA-E2-A1IU-01A-11R-A14D-07','TCGA-AO-A03U-01B-21R-A10J-07'))

getResults(query_TCGA)

# download data - GDCdownload
GDCdownload(query_TCGA)


# prepare data
tcga_brca_data <- GDCprepare(query_TCGA)

#And i get the following error message

leted after 0 s 
Error in `map()`:
i In index: 1.
i With name: 1.
Caused by error in `y[, 2:4]`:
! Can't subset columns past the end.
i Locations 2, 3, and 4 don't exist.
i There is only 1 column.
Run `rlang::last_error()` to see where the error occurred.
Warning messages:
1: One or more parsing issues, call `problems()` on your data frame for details, e.g.:
  dat <- vroom(...)
  problems(dat) 
2: One or more parsing issues, call `problems()` on your data frame for details, e.g.:
  dat <- vroom(...)
  problems(dat) 
3: One or more parsing issues, call `problems()` on your data frame for details, e.g.:
  dat <- vroom(...)
  problems(dat)
TCGAbiolinks • 2.0k views
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Entering edit mode

Hi, your code is working on my side. Please, what TCGAbiolinks version you have installed ?

enter image description here

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Entering edit mode

Oh, okay thank you so much. I checked and updated my version of R and BiocManager and now the code is working, what a rookie misstake. Thank you so much for the response.

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