Subsetting DESeq when different cell types
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@andrebolerbarros-16788
Last seen 1 day ago
Portugal

Hi everyone,

My question is more conceptual than practical. Right now, I am analysing differentially expressed genes in different immune cell types coming from different organs. I want to compare cell types within organs and also between organs (same type in different organs).

I expect there are some differences in the gene expression among cell types. So, in order to analyse cell types within the same organ - let's say B Cells vs T Cells in the spleen - should I subset the dataset? Or maintaining samples from other organs can add information?

Thanks in advance!

DESeq2 RNASeq • 504 views
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