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BioEpi • 0@11b02720
Last seen 3 days ago
Hello Mike, I have one question about the DESeq2 model. Given that I have 2 groups of RNA-seq, each of which has 3 biological replicates. Thus, each gene has 3 biological replicates in each condiction. does the DESeq2 model use average gene expression or each replicate of gene to estimate paratmeters, such as beta and intercept? Thanks
Hello Michael, thanks for your reply. Yes, I know that DESeq2 normalizes each gene in each sample. after normalization, we will have normalized expression level for each gene in each sample.
here is an example:
My question is: how does y(DESeq2) take in these normalized gene expression to build GLM? does DESeq2 use average normalized expression of each gene in each group or just use normalized expression of each gene in each group?
I am not sure whether my question is clear.