Cannot generate plots when calling ChAMP
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Entering edit mode
Kan • 0
@97877a7a
Last seen 12 months ago
Taiwan

Enter the body of text here

When I was calling DMP.GUI to use interactive visualization to see my differential methylated probe results, it came with an warning, which says "error in evaluating the argument 'x' in selecting a method for function 'sort' ", and I cannot see the results in the interactive view.

How can I solve this problem? Thanks a lot.

Code should be placed in three backticks as shown below


# include your problematic code here with any corresponding output 
myDMP <- champ.DMP(beta = myCombat,
                   pheno = myLoad$pd$Sample_Group,
                   adjPVal = 0.05,
                   adjust.method = "BH",
                   arraytype = "450K")

DMP.GUI(DMP = as.data.frame(myDMP[[1]]),
        beta = myCombat,
        pheno = myLoad$pd$Sample_Group) 
# please also include the results of running the following in an R session 
Listening on http://127.0.0.1:5420
<< Generating dmrplot >>
Warning: Error in <Anonymous>: error in evaluating the argument 'x' in selecting a method for function 'sort': 
  108: <Anonymous>
  107: stop
  106: Cutoff_repalce
  102: renderPlotly
  101: func
   98: shinyRenderWidget
   97: func
   84: renderFunc
   83: output$geneenrich
    2: runApp
    1: DMP.GUI

sessionInfo( )
R version 4.2.2 (2022-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Ventura 13.0.1

Matrix products: default
LAPACK: /Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] limma_3.54.0                               forcats_0.5.2                              stringr_1.5.0                             
 [4] dplyr_1.1.0                                purrr_1.0.1                                readr_2.1.3                               
 [7] tidyr_1.3.0                                tibble_3.1.8                               ggplot2_3.4.1                             
[10] tidyverse_1.3.2                            shiny_1.7.3                                doParallel_1.0.17                         
[13] ChAMP_2.28.0                               RPMM_1.25                                  cluster_2.1.4                             
[16] DT_0.26                                    IlluminaHumanMethylationEPICmanifest_0.3.0 Illumina450ProbeVariants.db_1.34.0        
[19] DMRcate_2.12.0                             ChAMPdata_2.30.0                           minfi_1.44.0                              
[22] bumphunter_1.40.0                          locfit_1.5-9.6                             iterators_1.0.14                          
[25] foreach_1.5.2                              Biostrings_2.66.0                          XVector_0.38.0                            
[28] SummarizedExperiment_1.28.0                Biobase_2.58.0                             MatrixGenerics_1.10.0                     
[31] matrixStats_0.63.0                         GenomicRanges_1.50.1                       GenomeInfoDb_1.34.4                       
[34] IRanges_2.32.0                             S4Vectors_0.36.1                           BiocGenerics_0.44.0                       

loaded via a namespace (and not attached):
  [1] rappdirs_0.3.3                                      rtracklayer_1.58.0                                 
  [3] R.methodsS3_1.8.2                                   wateRmelon_2.4.0                                   
  [5] ragg_1.2.4                                          bit64_4.0.5                                        
  [7] knitr_1.42                                          DelayedArray_0.24.0                                
  [9] R.utils_2.12.2                                      data.table_1.14.8                                  
 [11] rpart_4.1.19                                        KEGGREST_1.38.0                                    
 [13] RCurl_1.98-1.9                                      GEOquery_2.66.0                                    
 [15] AnnotationFilter_1.22.0                             generics_0.1.3                                     
 [17] GenomicFeatures_1.50.2                              preprocessCore_1.60.2                              
 [19] RSQLite_2.2.19                                      combinat_0.0-8                                     
 [21] bit_4.0.5                                           tzdb_0.3.0                                         
 [23] lubridate_1.9.0                                     xml2_1.3.3                                         
 [25] httpuv_1.6.9                                        assertthat_0.2.1                                   
 [27] IlluminaHumanMethylation450kmanifest_0.4.0          gargle_1.2.1                                       
 [29] viridis_0.6.2                                       IlluminaHumanMethylationEPICanno.ilm10b4.hg19_0.6.0
 [31] isva_1.9                                            xfun_0.37                                          
 [33] jquerylib_0.1.4                                     hms_1.1.2                                          
 [35] evaluate_0.20                                       missMethyl_1.32.1                                  
 [37] DNAcopy_1.72.3                                      promises_1.2.0.1                                   
 [39] fansi_1.0.4                                         restfulr_0.0.15                                    
 [41] scrime_1.3.5                                        progress_1.2.2                                     
 [43] readxl_1.4.1                                        dendextend_1.17.1                                  
 [45] dbplyr_2.2.1                                        DBI_1.1.3                                          
 [47] htmlwidgets_1.6.1                                   reshape_0.8.9                                      
 [49] googledrive_2.0.0                                   ROC_1.74.0                                         
 [51] ellipsis_0.3.2                                      crosstalk_1.2.0                                    
 [53] backports_1.4.1                                     permute_0.9-7                                      
 [55] annotate_1.76.0                                     biomaRt_2.54.0                                     
 [57] deldir_1.0-6                                        sparseMatrixStats_1.10.0                           
 [59] vctrs_0.5.2                                         ensembldb_2.22.0                                   
 [61] withr_2.5.0                                         cachem_1.0.6                                       
 [63] globaltest_5.52.1                                   Gviz_1.42.1                                        
 [65] BSgenome_1.66.3                                     checkmate_2.1.0                                    
 [67] GenomicAlignments_1.34.0                            prettyunits_1.1.1                                  
 [69] mclust_6.0.0                                        ExperimentHub_2.6.0                                
 [71] lazyeval_0.2.2                                      crayon_1.5.2                                       
 [73] genefilter_1.80.3                                   labeling_0.4.2                                     
 [75] edgeR_3.40.0                                        pkgconfig_2.0.3                                    
 [77] nlme_3.1-160                                        ProtGenerics_1.30.0                                
 [79] nnet_7.3-18                                         rlang_1.0.6                                        
 [81] lifecycle_1.0.3                                     nleqslv_3.3.4                                      
 [83] filelock_1.0.2                                      affyio_1.68.0                                      
 [85] BiocFileCache_2.6.0                                 modelr_0.1.10                                      
 [87] AnnotationHub_3.6.0                                 dichromat_2.0-0.1                                  
 [89] cellranger_1.1.0                                    rngtools_1.5.2                                     
 [91] IlluminaHumanMethylation450kanno.ilmn12.hg19_0.6.1  base64_2.0.1                                       
 [93] Matrix_1.5-3                                        Rhdf5lib_1.20.0                                    
 [95] reprex_2.0.2                                        base64enc_0.1-3                                    
 [97] googlesheets4_1.0.1                                 geneLenDataBase_1.34.0                             
 [99] viridisLite_0.4.1                                   png_0.1-8                                          
[101] rjson_0.2.21                                        bitops_1.0-7                                       
[103] R.oo_1.25.0                                         KernSmooth_2.23-20                                 
[105] rhdf5filters_1.10.0                                 blob_1.2.3                                         
[107] DelayedMatrixStats_1.20.0                           doRNG_1.8.6                                        
[109] qvalue_2.30.0                                       nor1mix_1.3-0                                      
[111] jpeg_0.1-10                                         scales_1.2.1                                       
[113] memoise_2.0.1                                       magrittr_2.0.3                                     
[115] plyr_1.8.8                                          zlibbioc_1.44.0                                    
[117] compiler_4.2.2                                      BiocIO_1.8.0                                       
[119] RColorBrewer_1.1-3                                  illuminaio_0.40.0                                  
[121] clue_0.3-64                                         Rsamtools_2.14.0                                   
[123] cli_3.6.0                                           DSS_2.46.0                                         
[125] affy_1.76.0                                         JADE_2.0-3                                         
[127] htmlTable_2.4.1                                     Formula_1.2-5                                      
[129] MASS_7.3-58.1                                       mgcv_1.8-41                                        
[131] tidyselect_1.2.0                                    stringi_1.7.12                                     
[133] textshaping_0.3.6                                   yaml_2.3.7                                         
[135] askpass_1.1                                         latticeExtra_0.6-30                                
[137] grid_4.2.2                                          sass_0.4.5                                         
[139] VariantAnnotation_1.44.1                            timechange_0.1.1                                   
[141] tools_4.2.2                                         rstudioapi_0.14                                    
[143] foreign_0.8-84                                      bsseq_1.34.0                                       
[145] gridExtra_2.3                                       farver_2.1.1                                       
[147] digest_0.6.31                                       BiocManager_1.30.19                                
[149] quadprog_1.5-8                                      Rcpp_1.0.10                                        
[151] broom_1.0.1                                         siggenes_1.72.0                                    
[153] BiocVersion_3.16.0                                  later_1.3.0                                        
[155] org.Hs.eg.db_3.16.0                                 httr_1.4.4                                         
[157] AnnotationDbi_1.60.0                                biovizBase_1.46.0                                  
[159] lumi_2.50.0                                         colorspace_2.1-0                                   
[161] rvest_1.0.3                                         fs_1.6.1                                           
[163] XML_3.99-0.13                                       splines_4.2.2                                      
[165] statmod_1.5.0                                       kpmt_0.1.0                                         
[167] multtest_2.54.0                                     shinythemes_1.2.0                                  
[169] systemfonts_1.0.4                                   plotly_4.10.1                                      
[171] xtable_1.8-4                                        jsonlite_1.8.4                                     
[173] marray_1.76.0                                       R6_2.5.1                                           
[175] Hmisc_5.0-1                                         pillar_1.8.1                                       
[177] htmltools_0.5.4                                     mime_0.12                                          
[179] glue_1.6.2                                          fastmap_1.1.0                                      
[181] BiocParallel_1.32.4                                 interactiveDisplayBase_1.36.0                      
[183] beanplot_1.3.1                                      codetools_0.2-18                                   
[185] utf8_1.2.3                                          bslib_0.4.2                                        
[187] lattice_0.20-45                                     sva_3.46.0                                         
[189] BiasedUrn_2.0.9                                     curl_5.0.0                                         
[191] gtools_3.9.4                                        GO.db_3.16.0                                       
[193] openssl_2.0.5                                       interp_1.1-4                                       
[195] survival_3.4-0                                      rmarkdown_2.20                                     
[197] methylumi_2.44.0                                    fastICA_1.2-3                                      
[199] munsell_0.5.0                                       rhdf5_2.42.0                                       
[201] GenomeInfoDbData_1.2.9                              goseq_1.50.0                                       
[203] impute_1.72.3                                       HDF5Array_1.26.0                                   
[205] haven_2.5.1                                         reshape2_1.4.4                                     
[207] gtable_0.3.1
ChAMP DMP.GUI • 380 views
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