how to solve this error
1
0
Entering edit mode
ashisaanz • 0
@c26258d6
Last seen 13 months ago
India

the data is Mature_miRNA_ID COV102 COV105 COV108 COV112 COV1 hsa-miR-548ap-3p 0 0 0 0 0 hsa-miR-548t-3p 0 0 0 0 0 hsa-miR-548e-3p 1 0 0 0 2 hsa-miR-548az-3p 0 0 0 0 0

for count data and metadata is id treatment COV102 test COV105 test COV108 test COV112 test COV1 test COV27 test COV33 test COV35 test

Code should be placed in three backticks as shown below

``` dds <- DESeqDataSetFromMatrix(countData=mycounts,colData=metadata,tidy=TRUE) converting counts to integer mode Error in DESeqDataSetFromMatrix(countData = mycounts, colData = metadata, : argument "design" is missing, with no default

``` how to solve this error

DESeq2 GEO miRNAData • 612 views
ADD COMMENT
0
Entering edit mode
ATpoint ★ 4.2k
@atpoint-13662
Last seen 15 hours ago
Germany

Please read the vignette to understand the very basics of how the software works. It will become clear then.

https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html

The design is the basis for DESeq2 to know what is shall compare and what to include into its model.

ADD COMMENT

Login before adding your answer.

Traffic: 760 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6