How does HTqPCR handle technical replicates?
Suppose I have 4 samples, 8 primer sets, and 3 replicates on a 96 well plate.
"n.features indicates the number of features present on each array."
Features are genes? So 8.
"n.data...Indicates the number of samples that are present in each file."
Samples are 4
"n.features*n.data must correspond to the total number of lines to be read from each file. "
8*4=32 But I have 96 lines of data?
Do I have to make each technical replicate it's own feature? How then would I indicate that they are replicates if they each have their own feature name? I can't reuse feature names because I can't reuse column names.
What am I not understanding?