DiffBind PE data
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rbronste ▴ 60
@rbronste-12189
Last seen 2.1 years ago

Hi,

In the current version of DiffBind what are the steps for indicating that PE data is being used? Thank you.

Rob

 

diffbind paired-end reads • 659 views
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Gord Brown ▴ 640
@gord-brown-5664
Last seen 11 months ago
United Kingdom

Hi,

DiffBind doesn't account for PE data.  (Yes, it should, but it doesn't.)  Either just use forward-strand reads, or make sure that all your data are PE, then it'll just count each end independently.

Cheers,

 - Gord

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Rory Stark ★ 4.2k
@rory-stark-5741
Last seen 6 hours ago
CRUK, Cambridge, UK

Updating this to reflect that DiffBind has supported PE data for a couple of years now.

The most recent version will automatically detect SE or PE from the bams and perform the counting appropriately.

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