Trouble reading samplesheet in with diffbind to create dba
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Mallory • 0
@fb560b0e
Last seen 1 hour ago
United States

Hello, I've been having a fair amount of trouble trying to create a dba object in diffbind from a .csv samplesheet. I used this code before, but I'm reanalyzing some data and I keep getting the error :

"Error in scan(file = file, what = what, sep = sep, quote = quote, dec = dec, : line 1 did not have 10 elements"

I'm not sure why it is expecting 10 columns. I checked the format to make sure my delimiters were correct and tried specifying sep = ",". I'm using narrowpeak format from MACS2 output. I also tripled checked my directories for my files to ensure my paths are accurate. Please see my code below along with the screenshot of my samplesheet attached. Any help would be greatly appreciated.

ATAC <- read.csv("ATACSox_samplesheet2023_Genrich05.csv")
SoxData <- dba(sampleSheet = ATAC)

enter image description here

sessioninfo() R version 4.4.0 (2024-04-24) Platform: aarch64-apple-darwin20 Running under: macOS Sonoma 14.5

Matrix products: default BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib LAPACK: /Library/Frameworks/R.framework/Versions/4.4-arm64/Resources/lib/libRlapack.dylib; LAPACK version 3.12.0

locale: 1 en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

time zone: America/Los_Angeles tzcode source: internal

attached base packages: 1 stats4 stats graphics grDevices utils datasets methods base

other attached packages: 1 lubridate_1.9.3 forcats_1.0.0 stringr_1.5.1 dplyr_1.1.4 purrr_1.0.2 readr_2.1.5 tidyr_1.3.1
[8] tibble_3.2.1 ggplot2_3.5.1 tidyverse_2.0.0 DiffBind_3.14.0 SummarizedExperiment_1.34.0 Biobase_2.64.0 MatrixGenerics_1.16.0
[15] matrixStats_1.3.0 GenomicRanges_1.56.0 GenomeInfoDb_1.40.1 IRanges_2.38.0 S4Vectors_0.42.0 BiocGenerics_0.50.0

loaded via a namespace (and not attached): 1 bitops_1.0-7 deldir_2.0-4 rlang_1.1.4 magrittr_2.0.3 compiler_4.4.0 png_0.1-8 vctrs_0.6.5 pwalign_1.0.0
[9] pkgconfig_2.0.3 crayon_1.5.2 fastmap_1.2.0 XVector_0.44.0 caTools_1.18.2 utf8_1.2.4 Rsamtools_2.20.0 rmarkdown_2.27
[17] tzdb_0.4.0 UCSC.utils_1.0.0 xfun_0.44 zlibbioc_1.50.0 jsonlite_1.8.8 DelayedArray_0.30.1 BiocParallel_1.38.0 jpeg_0.1-10
[25] irlba_2.3.5.1 parallel_4.4.0 R6_2.5.1 stringi_1.8.4 RColorBrewer_1.1-3 rtracklayer_1.64.0 SQUAREM_2021.1 limma_3.60.2
[33] numDeriv_2016.8-1.1 Rcpp_1.0.12 knitr_1.47 timechange_0.3.0 Matrix_1.7-0 tidyselect_1.2.1 rstudioapi_0.16.0 abind_1.4-5
[41] yaml_2.3.8 gplots_3.1.3.1 codetools_0.2-20 curl_5.2.1 hwriter_1.3.2.1 lattice_0.22-6 plyr_1.8.9 withr_3.0.0
[49] ShortRead_1.62.0 coda_0.19-4.1 evaluate_0.24.0 Biostrings_2.72.1 pillar_1.9.0 KernSmooth_2.23-24 generics_0.1.3 RCurl_1.98-1.14
[57] invgamma_1.1 truncnorm_1.0-9 emdbook_1.3.13 hms_1.1.3 munsell_0.5.1 scales_1.3.0 ashr_2.2-63 gtools_3.9.5
[65] glue_1.7.0 tools_4.4.0 apeglm_1.26.0 interp_1.1-6 BiocIO_1.14.0 BSgenome_1.72.0 locfit_1.5-9.9 GenomicAlignments_1.40.0 [73] systemPipeR_2.10.0 XML_3.99-0.16.1 mvtnorm_1.2-5 grid_4.4.0 bbmle_1.0.25.1 amap_0.8-19 bdsmatrix_1.3-7 latticeExtra_0.6-30
[81] colorspace_2.1-0 GenomeInfoDbData_1.2.12 restfulr_0.0.15 cli_3.6.2 GreyListChIP_1.36.0 fansi_1.0.6 mixsqp_0.3-54 S4Arrays_1.4.1
[89] gtable_0.3.5 digest_0.6.35 SparseArray_1.4.8 ggrepel_0.9.5 rjson_0.2.21 htmlwidgets_1.6.4 htmltools_0.5.8.1 lifecycle_1.0.4
[97] httr_1.4.7 statmod_1.5.0 MASS_7.3-61

DiffBind DifferentialPeakCalling • 222 views
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Entering edit mode
Anthony • 0
@48ee703f
Last seen 4 weeks ago
United States

I was struggling with this today too. For me it happened because my peak files were not in the correct format. The "10 elements" refers to the expected number of columns in a narrowPeak file, not the samplesheet. Check that your narrowPeak files have 10 columns, or change the peakCaller to "bed".

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Thank you for your reply! There mustve been something wierd going on with my peak formats as you pointed out. I recalled all the peaks and it worked.

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