Species level information couldn't find with DADA2 bioconductor package
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abhisek001 • 0
@6d5973d2
Last seen 12 days ago
India

Species cannot assigned to my data. Can anyone tell the problem?

```taxa <- assignTaxonomy(seqtab.nochim, refFasta = "/home/omic/Downloads/Final_analysis/fasta_files/rdp_train_set_18.fa.gz", minBoot = 80, tryRC = FALSE, outputBootstraps = FALSE, taxLevels = c("Kingdom", "Phylum", "Class", "Order", "Family", "Genus", "Species"), multithread = TRUE, verbose = TRUE)

taxa <- addSpecies(taxa, refFasta = "/home/omic/Downloads/Final_analysis/fasta_files/rdp_species_assignment_18.fa.gz", allowMultiple = FALSE, tryRC = FALSE, n = 2000, verbose = TRUE)

``` 0 out of 712 were assigned to the species level. Of which 0 had genera consistent with the input table.

Metagenomics dada2 MicrobiomeData • 138 views
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@basketball legends So anyone have any suggestions?

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