Deleted:Gene expression analysis of parents offsprings with multiple tissue types
1
0
Entering edit mode
s.malik • 0
@89fab1ac
Last seen 5 weeks ago
Pakistan

Hi, I am doing RNAseq analysis on various tissues such as placenta & cord blood, parent blood. There are case control groups (each around 40) based on health outcomes of infants and no biological duplicate of samples.

  1. After quantifying through salmon, I want to know what is the best approach for proceeding for Differentially expressed genes? I am doing separate analysis for each tissue type till the significant list of DEGs of a group and then comparison or correlation of these genes with each of the tissue type i.e., DEGs of case control from placenta with that of cord blood. Also I will compare them at functional levels.
  2. How can I compare gene expression pattern of parents with infants?
  3. can I process via DESeq2 as the dispersion for 10 pilot samples looks fine if I am not wrong.enter image description here

Thank you so much.

GeneExpression DESeq2 RNASeq • 151 views
ADD COMMENT
This thread is not open. No new answers may be added
Traffic: 544 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6