Normalization RNA-seq for multiple plates
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Pimpa • 0
@562af1b6
Last seen 1 day ago
United Kingdom

Hi, I'm sorry to be so naive in the bioinformatics world. I have sequenced my samples in multiple plates and now I do not understand the best way to normalise. I selected my samples from the plates, merged the samples and then proceeded to normalise using edgeR to minimise the effect between the plates. But I am not sure if this is the best way. Do you have any tips or articles that refer to this problem and explain the best way to normalise?

Thank you very much.

Normalization DESeq2 RNASeqData edgeR • 97 views
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What are "plates"? You mean sequencing runs? Have the libraries been prepared the same time or in a balanced / batch-aware fashion? Add some metadata to illustrate the layout.

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