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Question: analysis of biomarker data from elisa
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gravatar for abetz58
9 months ago by
abetz580
abetz580 wrote:

Hello,

I am new to bioconductor. Are there any package for the analysis of more than fifty serum markers by elisa. Did anybody ever apply limma to look at changes at the marker levels during treatment cycles relative to healthy controls. Any pointers are highly appreciated.

 

Andreas

ADD COMMENTlink modified 9 months ago by James W. MacDonald45k • written 9 months ago by abetz580
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gravatar for James W. MacDonald
9 months ago by
United States
James W. MacDonald45k wrote:

I don't think there are any ELISA specific analysis packages, because ELISA isn't usually considered a high-throughput type of analysis. You could hypothetically use limma for this sort of thing, but it would probably be reasonable to use trend =  TRUE and robust = TRUE in the call to eBayes because it's not clear that the variability of antibody binding will be as consistent as DNA hybridization.

ADD COMMENTlink written 9 months ago by James W. MacDonald45k
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