Off topic:Trouble adapting openCyto Transformation
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Rivka • 0
@rivka-11959
Last seen 6.7 years ago
Munich

Hello,

being still pretty new to OpenCyto I have been working through the bioconductor Tutorial and am now trying to reproduce the data output and plots with our data. I get an error trying to do the transformation part:

######################
# Transformation
chnls <- parameters(m_compMat)
transFuncts <- estimateLogicle(ncfs[[1]], channels = chnls)
ncfs_trans <- transform(m_ncfs_comp, transFuncts)

 

The error I get is:

error in logicle_transform(as.double(x), as.double(t), as.double(w), as.double(m),  : 
  Logicle Exception: DidNotConverge: scale() didn't converge

Unfortunately I have no idea what to look at exactly to solve this. Might there be a problem with the fcs files itself or maybe with our template? Can someone give me an idea? 

Thanks,

Rivka

 

 

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