Hello,
being still pretty new to OpenCyto I have been working through the bioconductor Tutorial and am now trying to reproduce the data output and plots with our data. I get an error trying to do the transformation part:
######################
# Transformation
chnls <- parameters(m_compMat)
transFuncts <- estimateLogicle(ncfs[[1]], channels = chnls)
ncfs_trans <- transform(m_ncfs_comp, transFuncts)
The error I get is:
error in logicle_transform(as.double(x), as.double(t), as.double(w), as.double(m), :
Logicle Exception: DidNotConverge: scale() didn't converge
Unfortunately I have no idea what to look at exactly to solve this. Might there be a problem with the fcs files itself or maybe with our template? Can someone give me an idea?
Thanks,
Rivka