Dear Bioconductor users,
I am working with RNA-seq data (raw counts) and I want to perform regularized cox regression modelling using glmnet package. First, I have performed VST transformation that makes RNA-seq data homoscedastic. Next do i have to set the argument of glmnet function standardize= TRUE for variable standardization (all variables to have unit variance) prior to fitting the model sequence and then use the resulting unstandardized coefficients to rank the selected features (genes) or in my case the default standardization is not appropriate ?
Thank you for your time in advance!!