**0**wrote:

I would like to use edgeR to perform an ANOVA for difference between multiple groups, but am wondering what the best way is to do this. I have an RNA-seq experiment with multiple groups that I modeled without an intercept term (because there is no control group). I find the ANOVA-like test described in the edgeR Useres guide (chapter 3.2.6 for LRT and 4.4.9 for QL F-test approach) very useful, but don't think it's applicable to a model without intercept. What I want to do is an ANOVA where the F-test would test the difference of each group means from grand means being zero. Is it possible to do such an analysis in edgeR? Would I need to define an additional intercept term for this? Please let me know if I didn't state my question clearly enough. Thank you very much in advance