Finding candidate binding sites for RNA binding proteins in R
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@walter-f-baumann-12439
Last seen 3.8 years ago

 

Hi,

I need to scan mRNAs for binding motifs of RBPs (RNA binding proteins) in human. 

After some research I came across this tutorial on how to find candidate binding sites of known transcription factors. So far I did not saw an implemented tool to directly work with RNA or RBPs.

I downloaded a file from CISBP-RNA containing information on some RNA binding proteins. The PWMs can be downloaded as txt files. I use Gencode GRCh37.p13. 

Now I want to search for potential binding sites per transcript and per 3'UTR (in RNA code). What is the most efficient way to procede? I downloaded all UTR et c. sequences using biomaRt, but it contains just the DNA sequences. I transcribed the sequence into RNA already manually, but then I can't use the matchPWM function anymore (it just works for DNAString objects). 

Is there also an matchPWM function for RNA sequences that is able to handle U instead of T? matchPWM is just able to handle DNA sequences. 

Thanks for help in advance!​

R bioconductor RNA mRNA RNA binding proteins • 785 views
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