Search
Question: How to determine means for rlog value of biological duplicates calculated by DESeq2
0
gravatar for JunLVI
14 months ago by
JunLVI40
Japan
JunLVI40 wrote:

Hi. I have used DESeq2 to performed differential analysis. 

And now I would like to plot the data using transformed values "rld", but not sure how to determine the means for the biological duplicates. The simplest way I could think of is use the arithmetic means. For normal log transformation, that would means using the geometric means of the biological duplicates. But even for that I am not sure that it is a legitimate way to do, let alone "rlog".

Any recommendation, thanks!

 

ADD COMMENTlink modified 14 months ago by Michael Love18k • written 14 months ago by JunLVI40
1
gravatar for Michael Love
14 months ago by
Michael Love18k
United States
Michael Love18k wrote:

hi,

I would take the simple arithmetic mean of rlog values if I wanted a "middle point".

ADD COMMENTlink written 14 months ago by Michael Love18k

@Michael Love, answer straightforward enough, thanks!

ADD REPLYlink written 14 months ago by JunLVI40
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.2.0
Traffic: 307 users visited in the last hour