I have trouble getting the goseq package to work.
I am using R-3.3.0; and have installed goseq version 3.5; as well as the "org.Hs.eg.db" package.
Unfortunately I don't get goseq functions to work:
Example:
ensgenes<-c("ENSG00000130303","ENSG00000124785","ENSG00000134138")
getgo(ensgenes,"hg19","ensgene",fetch.cats="GO:BP")
gives:
Error in getgo(ensgenes, "hg19", "ensgene", fetch.cats = "GO:BP") :
Couldn't grab GO categories automatically. Please manually specify.
any advice??
thanks
> sessionInfo()
R version 3.3.0 (2016-05-03)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu precise (12.04.4 LTS)
locale:
[1] LC_CTYPE=en_GB.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_GB.UTF-8 LC_COLLATE=en_GB.UTF-8
[5] LC_MONETARY=en_GB.UTF-8 LC_MESSAGES=C
[7] LC_PAPER=en_GB.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_GB.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] parallel stats4 stats graphics grDevices utils datasets
[8] methods base
other attached packages:
[1] rtracklayer_1.32.2 GenomicRanges_1.24.3 GenomeInfoDb_1.8.7
[4] org.Hs.eg.db_3.3.0 AnnotationDbi_1.34.4 IRanges_2.6.1
[7] S4Vectors_0.10.3 Biobase_2.32.0 BiocGenerics_0.18.0
[10] goseq_1.24.0 geneLenDataBase_1.8.0 BiasedUrn_1.07
[13] edgeR_3.14.0 limma_3.28.21 ape_4.1
[16] BiocInstaller_1.22.3
loaded via a namespace (and not attached):
[1] Rcpp_0.12.9 XVector_0.12.1
[3] zlibbioc_1.18.0 GenomicAlignments_1.8.4
[5] BiocParallel_1.6.6 lattice_0.20-34
[7] tools_3.3.0 SummarizedExperiment_1.2.3
[9] grid_3.3.0 nlme_3.1-131
[11] mgcv_1.8-17 DBI_0.6-1
[13] digest_0.6.12 Matrix_1.2-10
[15] bitops_1.0-6 biomaRt_2.28.0
[17] RCurl_1.95-4.8 memoise_1.1.0
[19] RSQLite_1.1-2 GO.db_3.3.0
[21] GenomicFeatures_1.24.5 Biostrings_2.40.2
[23] Rsamtools_1.24.0 XML_3.98-1.7