Question: Differential expression after different normalization methods
0
gravatar for Bio_Ram
2.6 years ago by
Bio_Ram0
Bio_Ram0 wrote:

Is it possible to use only the differential expression module of Deseq2 or any other method for miRNA isoforms expression?

I have normalized my data using RPM based normalization and looking to check the differential expression and log fold change. is there any methods so that i can test with more than one statistics to strengthen my analysis.

Thanks in Advance

 

ADD COMMENTlink modified 2.6 years ago by Michael Love26k • written 2.6 years ago by Bio_Ram0
Answer: Differential expression after different normalization methods
1
gravatar for Michael Love
2.6 years ago by
Michael Love26k
United States
Michael Love26k wrote:

Yes, I have heard of DESeq2 being used for differential microRNA expression, however, you should use counts instead of normalized expression values. This is discussed in the vignette.

ADD COMMENTlink written 2.6 years ago by Michael Love26k
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