Question: Differential expression after different normalization methods
gravatar for Bio_Ram
11 months ago by
Bio_Ram0 wrote:

Is it possible to use only the differential expression module of Deseq2 or any other method for miRNA isoforms expression?

I have normalized my data using RPM based normalization and looking to check the differential expression and log fold change. is there any methods so that i can test with more than one statistics to strengthen my analysis.

Thanks in Advance


ADD COMMENTlink modified 11 months ago by Michael Love17k • written 11 months ago by Bio_Ram0
gravatar for Michael Love
11 months ago by
Michael Love17k
United States
Michael Love17k wrote:

Yes, I have heard of DESeq2 being used for differential microRNA expression, however, you should use counts instead of normalized expression values. This is discussed in the vignette.

ADD COMMENTlink written 11 months ago by Michael Love17k
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