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Question: Differential expression after different normalization methods
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gravatar for Bio_Ram
5 months ago by
Bio_Ram0
Bio_Ram0 wrote:

Is it possible to use only the differential expression module of Deseq2 or any other method for miRNA isoforms expression?

I have normalized my data using RPM based normalization and looking to check the differential expression and log fold change. is there any methods so that i can test with more than one statistics to strengthen my analysis.

Thanks in Advance

 

ADD COMMENTlink modified 5 months ago by Michael Love14k • written 5 months ago by Bio_Ram0
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gravatar for Michael Love
5 months ago by
Michael Love14k
United States
Michael Love14k wrote:

Yes, I have heard of DESeq2 being used for differential microRNA expression, however, you should use counts instead of normalized expression values. This is discussed in the vignette.

ADD COMMENTlink written 5 months ago by Michael Love14k
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