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Question: Differential expression after different normalization methods
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gravatar for Bio_Ram
17 months ago by
Bio_Ram0
Bio_Ram0 wrote:

Is it possible to use only the differential expression module of Deseq2 or any other method for miRNA isoforms expression?

I have normalized my data using RPM based normalization and looking to check the differential expression and log fold change. is there any methods so that i can test with more than one statistics to strengthen my analysis.

Thanks in Advance

 

ADD COMMENTlink modified 17 months ago by Michael Love19k • written 17 months ago by Bio_Ram0
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gravatar for Michael Love
17 months ago by
Michael Love19k
United States
Michael Love19k wrote:

Yes, I have heard of DESeq2 being used for differential microRNA expression, however, you should use counts instead of normalized expression values. This is discussed in the vignette.

ADD COMMENTlink written 17 months ago by Michael Love19k
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