Question: fRMA normalization for Mouse Gene 2.1 ST Array
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gravatar for arisarkar88
2.5 years ago by
arisarkar880 wrote:

Hello,

          Can anyone help me with fRMA? I need to execute fRMA for "Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version]?

ADD COMMENTlink modified 2.5 years ago by Matthew McCall830 • written 2.5 years ago by arisarkar880
Answer: fRMA normalization for Mouse Gene 2.1 ST Array
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gravatar for Matthew McCall
2.5 years ago by
United States
Matthew McCall830 wrote:
Unfortunately, there are not frma vectors made for this platform. You could make your own frma vectors or use a related preprocessing algorithm (e.g. rma).
ADD COMMENTlink written 2.5 years ago by Matthew McCall830

Thank you..but I am a novice in R. Can you please help me regarding how to create frma vector for this case? 

ADD REPLYlink written 2.5 years ago by arisarkar880

As an R novice, I would not recommend undertaking the creation of your own frma vectors. It can be a fairly involved process, especially when you're dealing with a new platform. However, if you decide to proceed, I would start with this paper:

http://www.biomedcentral.com/1471-2105/12/369

And then look at the frmaTools package for some functions that will be useful when creating the frma vectors. 

 

 

 

ADD REPLYlink written 2.5 years ago by Matthew McCall830
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