How to do normalization between different enzyme libraries and test the difference by diffHic.
1
0
Entering edit mode
516356412 • 0
@516356412-13154
Last seen 4.3 years ago

For the param file are not the same of different enzyme libraries. How to combine two replilcates of two enzyme libraries into pairs. And how to do normalization between different enzyme libraries and test the difference by diffHic. 

 

diffhic • 423 views
ADD COMMENT
0
Entering edit mode
Aaron Lun ★ 27k
@alun
Last seen 19 hours ago
The city by the bay

This is not supported in diffHic, and there are no plans to do so. Rigorous integration of Hi-C data generated with different enzymes is challenging, because the interaction-level and genomic biases (dependent on cut site frequency, fragment length, etc.) will not cancel out between libraries. This means that the biases would have to be modelled explicitly, which requires strong assumptions to describe the process by which the biases are generated.

ADD COMMENT

Login before adding your answer.

Traffic: 254 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6