Question: DeSeq2 MA plot
gravatar for niu.shengyong
18 months ago by
niu.shengyong0 wrote:

I use the following codes and generate this MA plot. It looks quite weird for me. Is there any problem I should take care of? Thanks!

MA plot :

ddsHTSeq <- DESeqDataSetFromHTSeqCount(sampleTable=sampleTable, directory = directory, design=~condition)
colData(ddsHTSeq)$condition<-factor(colData(ddsHTSeq)$condition, levels=c('control','test'))


plotMA(dds, ylim=c(-2,2), main='M', alpha=0.1)




ADD COMMENTlink modified 18 months ago by Michael Love20k • written 18 months ago by niu.shengyong0
gravatar for Michael Love
18 months ago by
Michael Love20k
United States
Michael Love20k wrote:

The visual artifacts are typically when one group (here control) is sequenced at much lower depth than the treated group in a two group comparison. The patterns are unavoidable. It is not great to have large differences in sequence depth across the conditions, but I don't have a decision boundary or anything at which it's too much of a problem. I would zoom out on the plot (e.g. to [-6,6]) so you can see which genes are being called DE if any.

ADD COMMENTlink written 18 months ago by Michael Love20k
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