Bam file analysis fro beginner
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Morris ▴ 10
@morris-13226
Last seen 8 months ago
Italy

 

 

Hi everyone, 

I have a couple of bam files from RNAseq that I need to analyze.
I'm new to R world and so far I was able to install bioconductor and other packages for the analysis.
Could you guys give me links or tutorials including all the passages step by step, from the importation of bam files to how to make a differential expression analysis in R. 

I would appreciate your help 

thank you 

Patrizio 

bioconductor • 1.8k views
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@james-w-macdonald-5106
Last seen 3 hours ago
United States

How about this one?

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Or an alternative. You should probably read both.

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And for the second one (and maybe the first) you should also read this and this.

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Morris ▴ 10
@morris-13226
Last seen 8 months ago
Italy

Thank you for the useful links, 

I m following the first one, but when I try to import my external data "airway" package I have an error message.  

>  dir("bam files")
[1] "airway"              "Cell1.bam"          
[3] "Cell2.bam"           "RStudio-1.0.143.dmg"
> dir("airway")
character(0)
> indir <- system.file("extdata", package="airway", mustWork=TRUE)
Error in system.file("extdata", package = "airway", mustWork = TRUE) : 
  no file found

is it because I m not in the right directory?

Thanks 

 

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Yes. You want to point to the directory that contains your files. The code you are using is just a way for the vignette to work correctly and isn't intended to be taken literally.
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Morris ▴ 10
@morris-13226
Last seen 8 months ago
Italy

Hello,  

I was able to reproduce the workflow in the first link, and I have a better idea about the passages needed for the RNAseq analysis.

I have a couple of small bam files that I'd like to compare, but I can not find a way to convert this bam file in a package so that can be analyzed in R. 
How can I create a library containing my bam files? I can not find any tutorial 

Can you help me with that? 

Thank you 

P

 

 

 

 

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