Question: How to use Diffbind as complete beginner?
1
gravatar for AA
2.1 years ago by
AA10
AA10 wrote:

I am very new to bioinformatic analyses and have learned how to do the basic ChIP-seq workflow in Galaxy, including using Bowtie2 to map reads to the genome, MACS2 to call peaks, and doing motif searching.

Now I'd like to learn how to identify sites that are deferentially bound between two sample groups. I've read through the Bioconductor manual for Diffbind, but I do not understand much beyond the Introduction section.

I've installed R and RStudio, but am not knowledgeable in programming languages. I'm wondering how much I need to learn to be able to perform Diffbind on my ChIP-seq datasets.

Are there any Diffbind tutorials/guides for complete newbs?

diffbind chip-seq • 449 views
ADD COMMENTlink modified 2.1 years ago by Gord Brown590 • written 2.1 years ago by AA10
Answer: How to use Diffbind as complete beginner?
1
gravatar for Gord Brown
2.1 years ago by
Gord Brown590
United Kingdom
Gord Brown590 wrote:

DiffBind is an R package.  You can't expect to use it without a basic understanding of R.  If the vignette doesn't give you what you need, then you should consider taking an introductory R course.  Sorry, but there's no magic solution other than learning some R.

ADD COMMENTlink written 2.1 years ago by Gord Brown590

Great, thank you.

ADD REPLYlink written 2.1 years ago by AA10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 16.09
Traffic: 242 users visited in the last hour