How to use Diffbind as complete beginner?
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AA ▴ 10
@aa-13475
Last seen 11 months ago

I am very new to bioinformatic analyses and have learned how to do the basic ChIP-seq workflow in Galaxy, including using Bowtie2 to map reads to the genome, MACS2 to call peaks, and doing motif searching.

Now I'd like to learn how to identify sites that are deferentially bound between two sample groups. I've read through the Bioconductor manual for Diffbind, but I do not understand much beyond the Introduction section.

I've installed R and RStudio, but am not knowledgeable in programming languages. I'm wondering how much I need to learn to be able to perform Diffbind on my ChIP-seq datasets.

Are there any Diffbind tutorials/guides for complete newbs?

diffbind chip-seq • 671 views
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Gord Brown ▴ 640
@gord-brown-5664
Last seen 8 months ago
United Kingdom

DiffBind is an R package.  You can't expect to use it without a basic understanding of R.  If the vignette doesn't give you what you need, then you should consider taking an introductory R course.  Sorry, but there's no magic solution other than learning some R.

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Great, thank you.

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