correlation analysis

@Do-it!-23093

Last seen 4 hours ago

Germany, München

Hello,

I have RNAseq data from four groups(A, B, C, and D). I used DESeq 2 for Data normalization and transformation. Afterward, I was doing a pairwise correlation analysis for each group.
I had no problem performing the correlation analysis for A, B, and C. However, doing the fourth group(D) got a Warning message: **In sqrt(1 - h * h): NaNs produced**. For the correlation analysis, I used a CRAN software called **Hmisc**.
What would be the possible reason?

Best,
Amare

Hmisc
• 66 views
@u16406

Last seen 26 minutes ago

Republic of Ireland

This is not a question related to *DESeq2* and would be more suited to a general bioinformatics forum like Biostars.
Hint: check for missing or infinite values in your Group D data points. You should also always show the code that you have used and provide some sample input data such that the error can be reproduced.

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