I have some code which was working about a year ago. Now I have to re-use it and I get an error when running the following code:
fsa <- flowCore::read.flowSet( path = fcs_path, transformation = FALSE, phenoData = list( filename = "#SAMPLE", sample = "$SMNO", date = "$DATE", volume = "$VOL", proj = "$PROJ" ) )
Error in parse_pd_for_read_fs(files, path, pattern, phenoData, sep, as.is, : Argument 'phenoData' must be of type 'AnnotatedDataFrame' or a filename of a text file containing the phenotypic information
As I do not know much about flowcytometer and the file formats, I am completely at a loss what this error means. I understand it from the R side, but have no idea how I can solve it.
Also, I have no idea if this information is enough, or if more details are needed. If yes, please let ma know which ones and I can provide the info.
The data is from a BD Accuri C6 Plus. The Software is BD CSampler Plus Software Version 220.127.116.11.
Thanks a lot,
The following works:
fsa <- flowCore::read.flowSet( path = fcs_path, transformation = FALSE )